Kusamvana kwa ma subunits a thrombin ngati enzyme Agapurin kuchokera ku Agkistrodon halys venom ndikutsatizana kwa zotsalira za amino acid

[Mwatsatanetsatane] Cholinga Kudziwa zotsalira za N-terminal 15 amino acid zamagulu awiri ofanana a Agacutin.Magawo awiri a molekyulu ya enzyme amafunika kugawanika kuti apeze unyolo wa peptide wa subunit ndikutsatiridwa.Njira zoyeretsedwa kwambiri za Agacutin zidapezedwa pogwiritsa ntchito biochemical njira.Zitsanzo za subunit imodzi zozindikiritsa ndi kutsatizana zidakonzedwa ndi SDS-PAGE electrophoresis kupatukana, subunit adhesive strip recovery ndi PVDF membrane electrotransfer technology.Womalizayo adatsimikiza zotsalira za amino acid 15 pa N-terminal of subunit ndi Edman degradation method.Zotsatira Zotsatira za subunit yayikulu (16 kDa) inali DCSSGWSSYEEHQYY, ndipo yaing'ono (15 kDa) inali DSSGWSSYEGHEYYV.Pomaliza Zotsatira zotsatizana zidawonetsa kuti Agacutin anali thrombin yatsopano ya njoka ngati enzyme pofufuza database ya NCBI.