Kukonzekera ndi Kuphunzira kwa Ma antibodies a IgY ku Common Motif ya Metallase kuchokera ku Agkistrodon acutus Venom

Ma antibody a IgY motsutsana ndi zomwe zimachitika mu metalloproteinase kuchokera ku Agkistrodon acutus venom adakonzedwa ndipo gawo lake pakuchita zaululu wa njoka adaphunziridwa [Njira] Posanthula matsatidwe wamba a metalloproteinase osiyanasiyana kuchokera ku Agkistrodon acutus venom, tidapanga ndikupanga ma antigenic peptides PT2 otetezedwa kwambiri. , zomwe zimagwirizana kwambiri ndi ntchito zawo za enzyme;Nkhukuzo zidatetezedwa ndi ma conjugated complexes KLH-PT1 ndi KLH-PT2 kuti apeze ma antibodies a IgY.Titer ya IgY ndi mawonekedwe a mtanda ndi utsi wa Agkistrodon acutus ndi Agkistrodon brevius adaphunziridwa poyamba ndi ELISA ndi Western blot;Pomaliza, ntchito yochepetsetsa ya IgY idawunikidwa ndi kuyesa kwa anti subcutaneous hemorrhage mu mbewa [Zotsatira] ELISA ndi Western blot zidawonetsa kuti anti KLH-PT1 ndi anti Agkistrodon acutus venom IgY zitha kuwoloka ndi Agkistrodon acutus venom ndi Agkistrodon brevius venom, anali wamphamvu kwambiri kuposa woyamba;Anti KLH-PT2 IgY inalibe kutsutsana kwakukulu ndi Agkistrodon acutus ndi Agkistrodon brevius venoms in vitro, koma inawonetsa kawopsedwe amphamvu pakuyesa kutulutsa magazi mu vivo, ndipo inali yoposa awiri akale [Mapeto] The IgY antibody yomwe imatha. kuphatikizika ndi mavairasi osiyanasiyana a njoka ozungulira magazi kunakonzedwa popanga antigen peptide yomwe imagawidwa ndi metalloproteinases, yomwe idayala maziko okonzekera ma antibody enieni, ogwira mtima, otsika kawopsedwe komanso antihemorrhagic venom antibody.