Kukonzekera ndi Kuphunzira kwa Ma antibodies a IgY motsutsana ndi Common Base of Metallases kuchokera ku Agkistrodon halys Venom

[Abstract] [Cholinga] Kukonzekera ma antibody a IgY motsutsana ndi zomwe zimadziwika kuti metalloproteinase kuchokera ku Agkistrodon acutus venom, ndikuphunzira momwe zimakhudzira utsi wa njoka. ndi kupanga ma antigen peptides otetezedwa kwambiri PT1 ndi PT2, omwe amagwirizana kwambiri ndi ntchito ya enzyme;Nkhuku zimatetezedwa ndi mankhwala osakanikirana a KLH-PT1 ndi KLH-PT2 kuti apeze ma antibodies a IgY.Titer ya IgY ndi machitidwe okhudzidwa ndi Agkistrodon acutus ndi Agkistrodon acutus venom adaphunzira ndi ELISA ndi Western blot;Potsirizira pake, ntchito yowonongeka ya IgY inayesedwa ndi kuyesa kwa anti-subcutaneous hemorrhage mu mbewa [Zotsatira] Zotsatira za ELISA ndi Western blot zinasonyeza kuti anti-KLH-PT1 ndi anti-Agkistrodon acutus IgY akhoza kuwoloka ndi Agkistrodon acutus ndi Agkistrodon acutus venom. , ndipo womalizirawo anali wamphamvu kwambiri kuposa woyamba;Anti-KLH-PT2 IgY ilibe mgwirizano wodziwikiratu ndi Agkistrodon acutus venom ndi Agkistrodon acutus venom in vitro, koma ili ndi kawopsedwe kamphamvu pakuyesa koletsa magazi mu vivo, ndipo ndiyoposa ziwiri zakale [Mapeto] The IgY. Antigen peptide ya metalloproteinase yomwe ingathe kuwoloka ndi mavairasi osiyanasiyana ozungulira magazi inakonzedwa popanga ma antigen peptide ya metalloproteinase, yomwe inayala maziko okonzekera mankhwala enieni, ogwira ntchito, otsika kawopsedwe komanso odana ndi kutupa kwa njoka.