Pharmacokinetics ya thrombin kuchokera ku Agkistrodon acutus mu makoswe

[Abstract] Haemocoagulase Acutus (Halase), thermostatic yatsopano mu chitukuko cha halase, ndi thrombobin yogwira ntchito kwambiri ngati enzyme (TLE) yochokera ku njoka ya njoka ya Agkistrodon acutus Kuphunzira pharmacokinetics ya Halase, 125Ⅰ yolembedwa ndi radioisotope njira ndi radioassay yophatikizidwa ndi radioassay mpweya unayikidwa mu makoswe.Zotsatira zinawonetsa kuti milingo ya AUC inali yogwirizana bwino ndi Mlingo pansi pa njira zonse ziwiri zomwe zimagwiritsidwa ntchito, ndipo ma coefficients olumikizana anali 0.999 8 ndi 0.999 0 motsatana;Kuchuluka kwa mankhwala m'chiwindi, ndulu ndi mtima kunafika pachimake pambuyo pa mphindi 5 za makonzedwe, pomwe ma or-gans ena ambiri adafika pakatha mphindi 30, kenako pang'onopang'ono kuchepa;nthawi iliyonse, kuchuluka kwa mankhwala m'chiwindi kunali kokulirapo kuposa m'matumbo ena;Mafuta a Thrombin ochokera ku Agkistrodon acutus ndi mtundu wa thrombin wodzipatula komanso kuyeretsedwa ku poizoni wa Agkistrodon acutus.Ndi mtundu watsopano wa mankhwala a hemostatic opangidwa ndi gulu lathu The pharmacokinetics of thrombin kuchokera ku Agkistrodon acutus mu makoswe anaphunziridwa ndi 125 I-labeled radioisotope njira ndi trichloroacetic acid precipitation pamodzi ndi radioactive kuzindikira.Zotsatira zinasonyeza kuti ma AUC omwe amayezedwa ndi njira ziwirizo anali ogwirizana bwino ndi mlingo, ndipo ma coefficients ogwirizanitsa anali 0.9998 ndi 0.9990, motero;Mankhwala omwe ali m'chiwindi, ndulu ndi mtima anali apamwamba kwambiri a 5 min pambuyo pa makonzedwe, pamene kuti m'magulu ena ambiri anali apamwamba kwambiri a 30 min pambuyo pa makonzedwe, ndiyeno pang'onopang'ono anachepa;Pa nthawi iliyonse, mankhwala omwe ali m'matumbo a chiwindi anali apamwamba kwambiri kuposa omwe ali m'magulu ena;Kuphatikiza apo, kutuluka kwa thrombin kwa Agkistrodon acutus kumakhala kokwanira, makamaka ndi mkodzo.