Zotsatira za Chotupa Chopondereza Chigawo Choyamba cha Agkistrodon acutus Venom pa Migration Activity of Vascular Endothelial Cells

[Mwatsatanetsatane] Cholinga: Kuwona zotsatira za gawo loyamba la anti-chotupa la Agkistrodon acutus venom (AAVC-1) pa kusamuka kwa maselo a umbilical vein endothelial cell (HUVECs), ndikuwunika momwe AAVC - Ⅰ inhibiting. angiogenesis.Njira: Ma HUVEC adakulitsidwa mu vitro ndikuthandizidwa ndi AAVC - Ⅰ (0, 20, 40, 80 μ G / ml) maselo opangidwa ndi ma cell adayikidwa kwa 24 h.Kuyesa kukankha ndi kuyesa kwachipinda cha chemotactic kudagwiritsidwa ntchito kuti muwone momwe AAVC - Ⅰ pazochitika za endothelial cell migration;RT-PCR ndi Western blot adagwiritsidwa ntchito kuti azindikire kusintha kwa mRNA ndi mapuloteni a P-selectin ndi intercellular adhesion factor (ICAM-1) isanayambe komanso itatha mankhwala.Zotsatira: Poyerekeza ndi ma HU VEC mu gulu lodziwika bwino, kuthekera kwa kusamuka kwa maselo mu AAVC - Ⅰ magulu okhudzidwa adatsika mpaka madigiri osiyanasiyana, ndipo mawu a P-selectin ndi ICAM-1 mRNA adachepa kwambiri.Kutsiliza: AAVC - Ⅰ ikhoza kulepheretsa kusamuka kwa ma cell a endothelial mwa kuwongolera mRNA ndi mapuloteni a P-selectin ndi ICAM-1