Zotsatira za Inhibitory Component I ya Agkistrodon halys Venom pa Migration Activity of Vascular Endothelial Cells

Kuwona zotsatira za gawo lodana ndi chotupa I la Agkistrodon acutus venom (AAVC-1) pa kusamuka kwa maselo amtundu wa umbilical endothelial cell (HUVECs), ndikuwunika momwe angagwiritsire ntchito AAVC - Ⅰ inhibiting angiogenesis.Njira: Ma HUVEC adakulitsidwa mu vitro ndi AAVC - Ⅰ (0, 20, 40, 80 μ G/ml) maselo othandizidwa adakulungidwa kwa 24 h.Kuyesa kukankha ndi kuyesa kwachipinda cha chemotactic kudagwiritsidwa ntchito kuti muwone momwe AAVC - Ⅰ pazochitika za endothelial cell migration;RT-PCR ndi Western blot adagwiritsidwa ntchito kuti azindikire kusintha kwa mRNA ndi mapuloteni a P-selectin ndi intercellular adhesion factor (ICAM-1) isanayambe komanso itatha mankhwala.Zotsatira: Poyerekeza ndi ma HU VEC mu gulu lodziwika bwino, kuthekera kwa kusamuka kwa maselo mu AAVC - Ⅰ magulu okhudzidwa adatsika mpaka madigiri osiyanasiyana, ndipo mawu a P-selectin ndi ICAM-1 mRNA adachepa kwambiri.Kutsiliza: AAVC - Ⅰ ikhoza kulepheretsa kusamuka kwa ma cell a endothelial mwa kuwongolera mRNA ndi mapuloteni a P-selectin ndi ICAM-1