[Copy] Kudzipatula kwa zigawo za anticoagulant ndi fibrinolytic kuchokera ku Agkistrodon acutus venom

Kulekanitsa zigawo za anticoagulant ndi fibrinolytic kuchokera ku Agkistrodon acutus venom ndi zotsatira zake pa coagulation system.

Cholinga: Kuphunzira zotsatira za thrombin yoyeretsedwa ngati enzyme ndi plasmin kuchokera ku Agkistrodon acutus venom pamagazi a coagulation system.

Njira: Thrombin ngati enzyme ndi plasmin zidapatulidwa ndikuyeretsedwa kuchokera ku poizoni wa Agkistrodon acutus ndi DEAE Sepharose CL-6B ndi Sephadex G-75 chromatography, ndipo zotsatira zake pamilozera ya coagulation system zidawonedwa kudzera mu kuyesa kwa vivo.Results: Thrombin ngati enzyme ndi plasmin zidalekanitsidwa ndi chiwopsezo cha Agkistrodon acutus, ndipo zolemera zawo zamamolekyulu zinali 39300 ndi 26600, motsatana, Ma thrombin monga enzyme ndi plasmin kuchokera ku Agkistrodon acutus venom amatha kutalikitsa nthawi yonse yolumikizana ndi magazi, yolumikizidwa pang'ono. nthawi, nthawi ya thrombin ndi nthawi ya prothrombin, ndi kuchepetsa zomwe zili mu fibrinogen, koma zotsatira za thrombin ngati enzyme zimakhala zamphamvu, pamene plasmin imasonyeza zotsatira za anticoagulant pokhapokha pa mlingo wokulirapo, ndipo kuphatikiza kwa awiriwa kuli bwino kuposa ntchito yawo imodzi.

Pomaliza:

Thrombin ngati enzyme ndi plasmin yochokera ku Agkistrodon acutus venom imakhudza dongosolo la magazi mu nyama, ndipo kuphatikiza ziwirizi kumakhala ndi anticoagulant kwenikweni.