Antitumor invasion and metastasis of Agkistrodon acutus venom component ACTX-8 kumwera kwa Anhui Research on function

Cholinga Kufufuza zotsatira ndi gawo la chigawo cha Agkistrodon acutus venom ACTX-8 pa adhesion ndi kuwukira kwa khomo lachiberekero cell Hela

Shutdown system.Njira za Fibronectin zidakutidwa mu mbale ya chitsime cha 96.Maselo a Hela adadutsa 0, 2.5, 5, 10, 20, 40 g/ml ya ACTX-8

Pambuyo pa chithandizo, kugwirizana ndi Fibronectin kunadziwika, ndipo chiwerengero cha maselo otsatizana chinadziwika ndi MTY;Kuzindikira kuwukiridwa kwa ma cell ndi kusamuka ndi njira ya ma cell a TransweU

Kusintha kwa luso;Kumangirira pampikisano pakati pa ACTX-8 ndi Fibronectin ndi membrane ya cell ya Hela kudazindikirika ndi flow cytometry.Zotsatira:

Pambuyo pa chithandizo ndi ACTX-8, kumamatira kwa maselo a Hela ku Fibronectin kunachepa, ndipo kuyesa kwa maselo a transwell kunasonyeza kuti maselo a Hela anasamuka.

Kukhoza kusamuka ndi kuwukira kumachepetsedwanso.Zotsatira za cytometry zotuluka zikuwonetsa kuti ACTX-8 imatha kupikisana kuti imangirire cholandilira cha Fibronectin paselo.